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Journal: Bioactive Materials
Article Title: A self-locking conductive cardiac patch for immediate electrical integration with infarcted rat myocardium
doi: 10.1016/j.bioactmat.2025.10.045
Figure Lengend Snippet: The effects of patches on angiogenesis and inflammation in infarcted tissues 4 weeks post-MI. a) Immunofluorescence staining of α-smooth muscle actin (α-SMA, a vascular protein marker, green) and von Willebrand factor (vWF, an endothelial marker, red) of the infarct region in the MI, BMN-P, BMN-CP and CBMN-CP groups. b, c) Quantitative analysis of α-SMA (b) and vWF (c) in different groups based on fluorescent staining images (n = 4). d) Representative immunofluorescence staining of the infarct region in different groups for CD86 (green) and CD206 (red). e, f) Fluorescence intensity statistics of CD86 (e) and CD206 (f) in different groups based on fluorescent staining images (n = 4). Nuclei are stained blue with DAPI. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.
Article Snippet: The tissue sections were then incubated with the following primary antibodies: rabbit Connexin 43 primary antibody (BOSTER, BA1727, 1:200, China), mouse α-actinin primary antibody (Abcam, AB9465, 1:200, UK), mouse α-SMA primary antibody (Wuhan Sanying, 67735-1-IG, 1:400, China), rabbit vWF primary antibody (Wuhan Sanying, 27186-1-AP, 1:300, China),
Techniques: Immunofluorescence, Staining, Marker, Fluorescence
Journal: Materials Today Bio
Article Title: Vitamin C–functionalized copper nanozymes for treating drug-resistant intracellular infections and hyperinflammation
doi: 10.1016/j.mtbio.2025.102348
Figure Lengend Snippet: ROS- s cavenging and a nti- i nflammatory p roperties of CVs. (A). CLSM images showing ROS green fluorescent levels in RAW264.7 cells with DCFH-DA probe. Scale bar, 50 μm. (B). Representative images of flow cytometry detecting ROS with various groups in RAW264.7 cells. (C). Quantitative analysis of the mean fluorescence intensity of ROS depicted in B. (n = 3, mean ± s.d.). (D). Representative images of flow cytometry detecting apoptosis rates of RAW264.7 cells in different groups. (E). Quantitative analysis of the MFI of apoptosis rates depicted in D. (n = 3, mean ± s.d.). (F). Representative immunofluorescence images of CD86 in various groups. Scale bar, 50 μm. (G). Representative images of flow cytometry detecting CD86 with various groups in RAW264.7 cells. (H). Quantitative analysis of the MFI of CD86 depicted in G. (n = 3, mean ± s.d.). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Article Snippet: CD86 was detected using an
Techniques: Flow Cytometry, Fluorescence, Immunofluorescence